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Graduate Course Proposal Form Submission Detail - PHC6561
Tracking Number - 2775

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Current Status: Approved by SCNS - 2012-06-14
Campus: Tampa
Submission Type: New
Course Change Information (for course changes only):
Comments: To GC Chair 5/4/12 for MPH- Global Comm Conc. GC appd 5/15/12. to USf 5/15/12. to SCNS 5/23/12. Appd eff 8/1/12. Sub as 6569; Appd as 6561

Detail Information

  1. Date & Time Submitted: 2012-02-17
  2. Department: Global Health
  3. College: PH
  4. Budget Account Number: 640800
  5. Contact Person: Dr. Anupam Pradhan
  6. Phone: 8139747566
  7. Email:
  8. Prefix: PHC
  9. Number: 6561
  10. Full Title: Laboratory Techniques in Public Health
  11. Credit Hours: 3
  12. Section Type: L - Laboratory
  13. Is the course title variable?: Y
  14. Is a permit required for registration?: Y
  15. Are the credit hours variable?: N
  16. Is this course repeatable?:
  17. If repeatable, how many times?: 0
  18. Abbreviated Title (30 characters maximum): Laboratory Techniques in PH
  19. Course Online?: C - Face-to-face (0% online)
  20. Percentage Online: 0
  21. Grading Option: R - Regular
  22. Prerequisites: None
  23. Corequisites: None
  24. Course Description: This is a unique interactive laboratory based course. Each lecture will be supported by a “wet lab” where students would get hands on experience of laboratory research techniques using basic and advanced biochemical and molecular tools.

  25. Please briefly explain why it is necessary and/or desirable to add this course: Needed to compete with national trends
  26. What is the need or demand for this course? (Indicate if this course is part of a required sequence in the major.) What other programs would this course service? The overall goal of the proposed course is to expose graduate students to the rewards of laboratory research. By the end of the course, students will have a strong understanding of the fundamental laboratory techniques that will help them to advance in their chosen careers, whether as a researcher, or a public health worker directly engaged with their communities. There is no other course within the Global health curriculum that teaches students laboratory techniques.
  27. Has this course been offered as Selected Topics/Experimental Topics course? If yes, how many times? Yes, 2 times
  28. What qualifications for training and/or experience are necessary to teach this course? (List minimum qualifications for the instructor.)
  29. Objectives: By the end of the course, the students will develop a strong research understanding and will have “hands on experience” of the following:

    1. Principles of measurements, balances and pipetting, working of pH instrument, preparation of different media, buffers and indicators.

    2. General cell culture techniques, maintain cell cultures, viability and cytotoxicity assays.

    3. Microscopy, principles and applications. Preparation of slides using different fixatives and stains to observe and identify different organisms/cell organelles.

    4. DNA extraction, quantification, and separation by agarose gel. Primer designing and PCR amplification of their own genomic DNA by using buccal swabs.

    5. Basic principles and applications of colorimetry, spectrophotometry, and flowcytometry.

    6. Immunological techniques like western blot and ELISA.

    7. Protein estimation with Bradford and Lowry’s methods.

    8. Expression of foreign gene in bacterial host system, vector designing and recombinant protein expression, protein purification using Nickel-Agarose columns and analysis of purified protein by SDS-PAGE electrophoresis.

  30. Learning Outcomes: By the end of the course, students will have a strong understanding of the fundamental laboratory techniques that will help them to advance in their chosen careers, whether as a researcher, or a public health worker directly engaged with their communities.
  31. Major Topics: (1) Basic Laboratory Instrumentation, Quality control/GMP and GLP.(2)General Cell Culture Techniques. (3)Microscopy and chromatography. (4)Molecular Biology Methods (5)Photometry in Infectious Disease Research (cell sorting sorting using flow cytometer). (6)Immunolical techniques (ELISA). (7) Enzymology and Microbial Technology
  32. Textbooks: Required:

    1. Ninfa A.,Ballou,DP.,Benore,M.Fundamental Laboratory Approaches for Biochemistry and Biotechnology.John Wiley & Sons,Inc.,Hoboken,New Jersey.

  33. Course Readings, Online Resources, and Other Purchases: Recommended Books:

    1.Pollenz RS.,Kimble,M.Cannons,A.Experiments in Cell Biology.Kendall/Hunt Publishing Company,Dubuque,Iowa.

    2.McPherson J.,Quirke P.,Taylor G.R.PCR-A Practical Approach.Oxford University Press, Oxford,England.

    3.Sambrook and Russel.Molecular Cloning,A Laboratory Manual,Vol.1-3.Cold Spring Harbor Laboratory Press.NewYork.

    4. Lab Math: A Handbook of Measurements, Calculations, and Other Quantitative Skills for Use at the Bench

    Dany Spencer Adams, The Forsyth Institute

    Online Resources:

    Additional resources is expected from class assignments, and additional readings maybe assigned and/or recommended.

    All lab supplies will be provided, except that the students will be responsible to buy their own lab coats (white only) and calculators. Student will not be permitted to attend the class without a lab coat. Sandals and shorts cannot be worn in the lab. Due to security reasons students will be required to leave their cell phones outside the building, if equipped with cameras.

  34. Student Expectations/Requirements and Grading Policy: Lab Reports 100%.
  35. Assignments, Exams and Tests: Students are advised to read the posted lectures and the assignment before they come to the class. Handouts will be provided but they are required to bring their own lab coats, punch registers and calculators.

    1. The students will be evaluated on the basis of the lab reports (100% course grade).

    2. The Lab Reports are based on the assignments performed on each Wet Lab Session and is due 6:00 pm next class. Each Lab Report earns a maximum of 10 points which is subsequently accumulated over the progression of the course and converted to 100%. The converted percentage is then graded and the scheme is as follows:

    95-100= A+


  36. Attendance Policy: This course has overall 13 lectures divided into two sections, 1-7 and 9-14. At the end of the first section 1-7 there is a group based paper presentation on a novel lab based research technique (open to public for extra credit). The students will be required to turn in their lab reports every week which is the only scoring pattern.

    A 100% attendance is necessary. All wet lab reports are graded following submission of the reports on timely basis. One session skipped equals to loss of 10 points which can account directly to the total course percentage and the grading.

    Course Attendance at First Class Meeting – Policy for Graduate Students: For structured courses, 6000 and above, the College/Campus Dean will set the first-day class attendance requirement. Check with the College for specific information. This policy is not applicable to courses in the following categories: Educational Outreach, Open University (TV), FEEDS Program, Community Experiential Learning (CEL), Cooperative Education Training, and courses that do not have regularly scheduled meeting days/times (such as, directed reading/research or study, individual research, thesis, dissertation, internship, practica, etc.). Students are responsible for dropping undesired courses in these categories by the 5th day of classes to avoid fee liability and academic penalty. (See USF Regulation – Registration - 4.0101,

    Attendance Policy for the Observance of Religious Days by Students: In accordance with Sections 1006.53 and 1001.74(10)(g) Florida Statutes and Board of Governors Regulation 6C-6.0115, the University of South Florida (University/USF) has established the following policy regarding religious observances: (

    In the event of an emergency, it may be necessary for USF to suspend normal operations. During this time, USF may opt to continue delivery of instruction through methods that include but are not limited to: Blackboard, Elluminate, Skype, and email messaging and/or an alternate schedule. It’s the responsibility of the student to monitor Blackboard site for each class for course specific communication, and the main USF, College, and department websites, emails, and MoBull messages for important general information.

  37. Policy on Make-up Work: Medical emergencies or absence which cannot be avoided should be communicated to the Instructors at the earliest possible. However, missed class (only wet lab) can be scheduled in communication with the course instructors at their convenience within a week to learn and become eligible to submit a lab report. (Also see Institutional Policies section for Emergency Preparedness for Academic Continuity)
  38. Program This Course Supports: Public Health, Global Health
  39. Course Concurrence Information: Chemistry, Biology, Medicine and Allied Sciences.

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