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Graduate Course Proposal Form Submission Detail - BSC5420

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Current Status: Approved, Permanent Archive - 2009-03-04
Submission Type:
Course Change Information (for course changes only):

  1. Department and Contact Information

    Tracking Number Date & Time Submitted
    1654 2007-04-20
    Department College Budget Account Number
    Biology AS 120901000
    Contact Person Phone Email
    RS Pollenz 9741596 pollenz@cas.usf.du

  2. Course Information

    Prefix Number Full Title
    BSC 5420 Genetic Engineering and Recombinant DNA Technology

    Is the course title variable? N
    Is a permit required for registration? Y
    Are the credit hours variable? N
    Is this course repeatable?
    If repeatable, how many times? 0

    Credit Hours Section Type Grading Option
    3 C - Class Lecture (Primarily) R - Regular
    Abbreviated Title (30 characters maximum)
    Gen Eng DNA Tech
    Course Online? Percentage Online


    PCB3023, PCB3063, either PCB3023L or PCB3063L


    Course Description

    This lecture-based course will use a problem solving approach provide fundamental knowledge of scientific concepts and principles that form the basis of experimental methodologies in genetic engineering and recombinant DNA technology for majors/nonmajors

  3. Justification

    A. Please briefly explain why it is necessary and/or desirable to add this course.

    This graduate level (5000) course has been offered 5 times since the Spring 2003 semester and has become an integral part of the course work for the MS and PhD degrees in Biology for students specializing in cell biology and molecular biology. This cours

    B. What is the need or demand for this course? (Indicate if this course is part of a required sequence in the major.) What other programs would this course service?

    This graduate-level course has serviced nearly all the incoming graduate students specializing in cell biology and molecular biology within the CMM Division of the Department of Biology since it was first offered in the spring of 2003. It has also been taken by numerous advanced undergraduate as well as post baccalaureate students who are preparing for advanced study in graduate and medical degree programs. The course currently is not required for incoming graduate students, but as various graduate "tracks" are developed within the CMM Division, this course will become one of four courses that make up a required core.

    C. Has this course been offered as Selected Topics/Experimental Topics course? If yes, how many times?

    Yes, the course has been offered 5 times and has had an average enrollment of 21 students (both graduate and advanced undergraduate)

    D. What qualifications for training and/or experience are necessary to teach this course? (List minimum qualifications for the instructor.)

    PhD degree in a biological science with laboratory experience in recombinant DNA technology and experimental methods in molecular biology.

  4. Other Course Information

    A. Objectives

    The explosion of biotechnology over the past decade is truly remarkable. Even now in 2007, it is difficult to keep up with the new techniques and automation that allows the manipulate DNA sequences. However, behind all the technology and the various “kits” that allow complicated gene manipulations to be carried out in a matter of hours, are several less complicated concepts. The goal of this course is to explore the “basic techniques” of manipulating genes and proteins to provide the student sufficient background to design and interpret experiments dealing with genetic engineering.

    B. Learning Outcomes

    It is expected that the student who successfully complete the course will have complied a database of experimental procedures as well the ability to design, carry out, and troubleshoot them as well as analyze the procedures that are detailed in scientific manuscripts. Assessment will be through applied, written examinations that require hypothesis testing and written descriptions of experimental paradigms using the various techniques discussed in the course.

    C. Major Topics

    In this course students will be exposed to: 1) the use of restriction enzymes, plasmids, phage and viruses to generate recombinant DNA molecules, 2) generation and screening of libraries, 3) use and analysis of PCR techniques, 4) recombinant proteins expression and generation of antibodies, 5) modulation of gene regulation use siRNA and other techniques and 5) generation and analysis of transgenic animals.

    D. Textbooks

    Recombinant DNA, (2007) James Watson et al., WH Freeman (3rd Edition) ISBN-10 071672866-4

    E. Course Readings, Online Resources, and Other Purchases

    F. Student Expectations/Requirements and Grading Policy

    G. Assignments, Exams and Tests

    H. Attendance Policy

    I. Policy on Make-up Work

    J. Program This Course Supports

  5. Course Concurrence Information

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